Methanolic seed extracts of peganum harmala exhibit potent. The main protease of sarscov2, 3clike protease 3clpro, is highly conserved across coronaviruses and is essential for the maturation process of viral. International journal of infection antifungal effect of. Effectiveness of using oils extracts of peganum harmala and. Feng and kang 1994 showed that the ethanolic extract of peganum harmala seeds gave a stronger scolicidal effect at 2% concentration, as the mortality rate of protoscolices were reaches 90%, and they conclude that the scolicidal activity of this extract may be due to the presence of alkaloids. Further, results of their antioxidant screening revealed that aqueous extract with ic 50 values of 111. It is also effective in the treatment of dermatosis, hypothermia, and cancer.
Scientific research and essays effectiveness of using. Alkaloids were isolated from peat and its extract for the first time. In present study the effects of methanolic extract of peganum harmala l. Phytochemical analysis of leaf extract of phyllanthus fraternus. As is the case with taxol, harmala alkaloids are difficult to synthesize.
There is an urgent call for developing effective drugs against the virus sarscov2 causing this disease. Synthesis of silver nanoparticles using peganum harmala. In particular, herbal remedies attract considerable research interest because of their less adverse effects comparing with synthetic drug treatments. The rf value of the different compounds present in the extract was found to be 0. While the composition of the sea force 2 which extract of seaweed mixture with trace elements are. Effect of inhalation alcoholic extract of peganum harmala. This difference in mortality rate is due mainly to the variation in the contents in the various organs of the plant more important in seeds than leaves. Male rats weighting 200250 g were divided into 5 groups. They were subsequently sundried and ovendried gallenkamp, uk at 40 0c till a constant weight was achieved. Phytochemical screening and evaluation of the diuretic. Identification of phytoconstituents is done by performing following tests. Phytochemical analysis of methanolic extracts of leaves of.
The percent change in mean inhibition zone diameter attributed to the effect of extract was computed. The comparative effect of contact and ingestion tests between the two bioactive extract by their respective ld 50 show that seeds aqe are more effective than leaves. The biological activity of waterethanol extracts from various types of peat were studied. Peganum harmala seeds alcoholic extract at 75 and 100 mg kg caused slight to moderate histological changes in the cortex and medulla manifested as degeneration and hypertrophy of tubular. Antibacterial activity of espand peganum harmala alcoholic extracts against six pathogenic bacteria in planktonic and biofilm forms zeinab mohsenipour m. Feb 11, 2020 current drugs used against hsv1 and hsv2.
Zygophyllaceae fresh seeds were collected from the yellapur region, in february 1998 obtained through ms. Antimicrobial effects of aqueous and alcoholic extract of peganum harmala 5 powder was kept in a tightly closed glass container in refrigerator until its used to prepare different concentrations. The objective of this work is insufficient information on the study of plants p. Phytochemical screening, total phenolics and biological. Peganum harmala, commonly called wild rue, syrian rue, african rue, esfand, or harmel, among other similar pronunciations and spellings, is a perennial, herbaceous plant, with a woody underground rootstock, of the family nitrariaceae, usually growing in saline soils in temperate desert and mediterranean regions. Zeinab eltahir bakheet eltahir, abdelhafiz adam dahab. Pdf effect of repeated administration of peganum harmala. Banerjee 1, 1department of physiology, indian institute of chemical biology, 4, raja s. Tamarind seed extract enhances epidermal wound healing. Newer agents like ivabradine, and zatebradine are known to. Development of a two cm layer of foam indicated the presence of saponins. The animals were sacrificed on day 10 for histopathologic and biomechanical examinations. Medicinal plants extracts with antiangiogenic activity.
In this study different extracts of peganum harmala. All experiments were repeated at least three times in duplicate. Experimental and computational study of biological. Alcoholic extract preparation the crude extract was obtained according to subhani et al. All the natural plant extracts mentioned above, especially p. Thus, the aim of this study was to extract and purify protein from the seeds of p. About 50 mg of plant extract was diluted with distilled water and made up to 20 ml.
To these 3 drops of concentrated sulphuric acid was added. Hilgreen company, bangalore, in march 1998 and authenticated by our pharmacognosy department where a voucher specimen ame02 is. Pdf biological activities of peganum harmala leaves. It is effective for the treatment of dermatosis disease 9, its leaves are used as an antinociceptive 10. The plant extract contains alkaloids like morphine and boldine.
Study of the chemical components of peganum harmala and. Test for proteins to 2 ml of plant extract, 4 drops of 0. The suspension was shaken in a graduated cylinder for 15 min. Antibacterial activity of some plant extracts against some. Phytochemical analysis and evaluation of antioxidant. December 27, 2007 abstract harmala alkaloids are a common plant extract with a number of.
Chemical composition and antibacterial properties of peganum. Effect of ethanolic extract and crude alkaloides of. Antibacterial activity of some plant extracts against some bacterial species abdalnabi j. For the preparation of the filmforming solution, the gelatin was hydrated at room temperature 25 2 c for 30 minutes, followed by dissolution at 55 c for. Harmala alkaloids are schedule 9 drugs under poisons standard of december 2019.
The extract was run along with the standard extract showed the presence of presence of flavanoids and it was confirmed from the chromatogram after derivatization. Phytochemical analysis and cardiac depressant activity of. Celosia argentea linn leaf extract, planta indica 2. Experimental and computational study of biological activities. While in the case of mbc, it was discovered that a higher concentration of 100mgml is needed for both extract to be able to kill the tested microorganisms, except in the case of streptococcus pyogenes and candida. Scutellaria baicalensis extract and baicalein inhibit. Covid19 has become a global pandemic that threatens millions of people worldwide. Phytochemical analysis and antibacterial activity of psidium. Pdf pharmacological and therapeutic effects of peganum. If allelopathy, where a species produces toxic and affects the other, we see that the species can be made to disappear if the dose of these substances appears high. Biological control test of ethanol extracts of peganum. Ccl 4 was induced in 42 male rats for eight weeks and checked the protective efficacy of methanolic extract of carissa opaca leaves at various hormonal imbalances, alteration of antioxidant enzymes, dna fragmentation levels and lipid peroxidation caused testicular fibrosis in testis while high performance liquid chromatography hplc was used for detection of bioactive components.
Department of biology, faculty of science, university of hail, hail, kingdom of saudi arabia. However, there was no significant change in the ph of urine samples of the extract treated group compared with control. Biological activity of waterethanol extracts from peat and. Variations in biological attributes and phenolics of enzymatically hydrolyzed medicinal plant extracts. Blackwell publishing ltdhypolipidaemic effect of s. Evaluation of the genetic variability among a wild peganum. Extraction, characterization and biological studies of. Rao, phytochemical analysis of ethanolic extract of leaves of clerodendrum viscosum eelcv. The harmala alkaloids temporarily prevent biogenic amine from binding to the active site of the mao molecule and undergoing deamination. Tables 1 and 2 shows that humic acid caused significant differences in all shoot characteristics as compared to the control. Recent years of research has demonstrated different pharmacological and therapeutic effects of p.
The dried leaves were milled thomaswiley machine, england and the sieved powdered sample kept in stopperred glass bottles till needed. Extract reduces oxidative stress and improves symptoms in 6hydroxydopamineinduced parkinsons disease in rats maryam rezaeia, sima nasri b, mehrdad roughanic, zeinab niknami and seyed ali ziaid arazi herbal medicines research center, lorestan university of medical sciences, khoramabad, iran. Antileishmanial activity of peganum harmala extract on the. Effect of carissa opaca leaves extract on lipid peroxidation. The aphrodisiac properties of the methanol extract of leaves of passiflora incarnata linn. Test for steroid a small amount of aqueous extract of sample and a few crystal of sodium nitrate were taken in a dry test tube and heated gently for a minute. Harmala alkaloids and their natural sources are legal in most parts of the world. Biswas r a, a dasgupta, s k mitra, p k p roy, b dutta, s achari and t k chatterju, 2005.
Extraction, physicochemical, phytochemical analysis and. The extract was then applied once daily on half of the wounds. Medicinal plants effective on premature ejaculation. Phytochemical and hptlc studies of methanolic extract of.
The xrd pattern of the silver nanoparticles shows intense peaks at the 2. Evaluation of aqueous leaves extract of moringa oleifera linn. The effect of extraction protocol on the phytochemical and. Phytosterols test small portion of the plant extract was dissolved in chloroform. Essential oils extracted from two medicinal plants peganum harmala and rhanterium epapposum native to the arabian peninsula specially saudi arabia, were evaluated for their larvicidal effects and emergence of khapra beetle trogoderma granarium adults. Results and discussion 1vegetative characteristics. Peganum harmala l extract reduces oxidative stress and. To determine the effect of ph, the methanolic extract of the most effective.
Our study revealed that total alkaloids from seeds of the medicinal plant peganum harmala l. Amongst these, the highest activity was observed with. The oil analyzed by thin layer chromatography, the results of phytochemical analysis indicated that the. On the basis of present investigation the extract showed high significance p extract of the pods of moringa oleifera is a good source of compounds with antioxidant properties while the extract also exhibited significant free radical scavenging activity, reducing power activity and total antioxidant. The medicinal herb peganum harmala l zygophyllaceae. Pdf effectiveness of using oils extracts of peganum harmala and. Phytochemical analysis and antibacterial activity of moringa. The impact of terpenoids isolated from seeds of peganum. Some commonly known antiherpetic drugs that are currently being used include acyclovir acv, penciclovir, and famciclovir, which inhibit hsv1 and hsv2 infection by interfering with the viral dna polymerase and hence, viral genome replication kukhanova et al. Hexanic extracts by soxhlet and by maceration, ethyl acetate extracts, methanolic extracts, aqueous extract. These findings indicated that the extract could have compounds similar to ivabradine, that cause bradycardia in frog heart. Presence of greenish blue colour indicates the presence of glycosides.
Meliadubia also called as maha neem or forest neem is a high value medicinal plant of india, srilanka. Biology and biotechnology department herbarium of the same university. Moreover, harmane has been shown to decrease nfkb, mmp2, and mmp9 expression. Aphrodisiac activity of methanol extract of leaves of. Insecticidal potency of aqueous extracts of seeds and leaves.
Pbs extract was the most effective in increasing the rate of healing. Harmala alkaloids are shortterm monoamine oxidase inhibitors. The virus extract mixtures were kept at 4oc for 1hr to react. Dpph radical scavenging activity of peganum harmala seed extracts obtained by soxhlet a or maceration b extraction, and activity of the hexanic extracts obtained by either of both methodsc. Extraction, identification, and quantification of harmala.
Control, neurotoxin injection of 6hydroxydopamine into left hemisphere substantia nigra, peganum. Proximate, antinutritional and phytochemical composition of. Pharmacological and therapeutic effects of peganum harmala. Assessment of biochemical, phytochemical and antioxidant activities of eight mangrove plant leaf extracts european academic research vol. Phytochemical analysis and antiviral potential of aqueous.
Essential oils extracted from two medicinal plants peganum harmala and. Antimicrobial effects of aqueous and alcoholic extract of. Pdf tamarind seed extract enhances epidermal wound healing. Smoke and extract of total alkaloids were investigated for antimicrobial activity. In this study, the results showed that with a concentration increase of plants extract or drug, while. An maoi acts to inhibit a key enzyme, monoamine oxidase mao, in the human body, responsible for processes in the brain and throughout the body. To 5ml of extract solution, 1ml of lead acetate solution was added. Antimicrobial effects of aqueous and alcoholic extract of peganum harmala 7 such a difference was attributed to the effect of extract at the tested concentration compared to chx control. Studies on phytochemical, nutritional analysis and screening. Alcoholic extracts of the aerial parts and seeds of peganum harmala and teucrium polium were prepared using standard methods. Phytochemical and biological activities of four wild.
The extract is filtered by using whatman filter paper. Dr s subramanian, department of biochemistry and molecular biology, university of madras, guindy campus, chennai 600 025, tamil nadu, india. It has also been shown in various pharmacological studies that p. Extract also contains tannins, saponin, terpenoid and steroid. However, harmala alkaloids are easy to extract from species of passion flowers. The current research included testing the effectiveness of some plant extracts against insect cotton, so prepare extract terpenoids from harmala seeds and leaves of oleander plant. Antibacterial activity of peganum harmala sciencedirect. The results of our study on these four plants revealed that the examined fruits were a valuable source of nutraceuticals and exhibited good level of. Effectiveness of using oils extracts of peganum harmala.
At present, there are numerous antiviral drugs for the treatment of hsv infections. The fruits of four wild plants, namely, capparis decidua, ficus carica, syzygium cumini, and ziziphus jujuba, are separately used as traditional dietary and remedial agents in remote areas of khyber pakhtunkhwa, pakistan. Mullick road, kolkat a 700 032, india 2deptartment of. Current antivirals and novel botanical molecules interfering. Potential uses and analysis of bioactive natural compounds. Ii, issue 9 december 2014 11980 assay of catalase catalase activity was determined by the titrimetric method chance and maehly, 1995. Its effect should be considered in the context of its extensive usage in the men daily life. Harmala alkaloids except in herbs, or preparations, for therapeutic use. A low larvicidal activity was recorded for a concentration of 1. Darabpour, the result showed that mic and mbc values for the seed and root extract of p.
Antimicrobial activity of harmal plant peganum harmala l. Assessment of biochemical, phytochemical and antioxidant. Inoculation of eggs ninedayold embryonated chicken eggs were divided into ten groups of fives. Effect of the addition of soy lecithin and yucca schidigera 2. Study on phytochemical screening, hplc analysis of phenols. The present study investigated the antimicrobial activity. Mathematical modeling of the allelopathic effect of leaf. Study of plant diversity of karnal district, haryana, india. Purification of antioxidant protein isolated from peganum. The characteristic peaks obtained in the xrd pattern are shown in figure 3. It was found that the biological activity of the alkaloids was higher than that of the waterethanol extracts. The extract 100 and 50 mgkg significantly p extract administration. To 2 ml of plant extract, 1 ml of glacial acetic acid and 5% ferric chloride was added.
Experimental and computational study of biological activities of alkaloids isolated from peganum harmala seeds f. Activity of peganum harmala extract against antibiotic. Isolation purification and characterization of four pure compounds from the root extract of pluchea indica l less and potentialities of the root extract and. Synthesis of silver nanoparticles using peganum harmala extract as a green route matin azizi a, sajjad sedaghat b, kambiz tahvildari a, pirouz derakhshi a and ahad ghaemi c a departmentofchemistry,collegeofscience,northtehranbranch,islamicazaduniversity,tehran,iran. This model has been treated while putting into consideration the abiotic conditions and the number of repetitions in the plot factors. Studies on phytochemical, nutritional analysis and screening of in vitro biological activities of meliadubia leaf extract g. Isolation, phytochemical and biological evaluation of acacia. Peganum harmala seeds alcoholic extract at 75 and 100 mg kg caused slight to moderate histological changes in the cortex and medulla manifested as. Monodora myristica african nutmeg seed oil contain important compounds such as arachidonic, oleic, pinene and linoleic acid, which can be used industrially for the. Flocculent brown precipitate indicates the presence of tannins. Biological actions and medicinal applic ations ishita chattopadhyay 1, kaushik biswas 1, uday bandyo p adhyay 2 and ranajit k. The pulp of freshly harvested mature synsepalumdulcificum miracle fruit berries were analyzed for proximate, ph, titratable acidity, antinutritional and phytochemical content. College of science, alqadisiyah university, iraq abstract. Department of biology, faculty of science, shahid chamran.
The our results showed that there was no significant difference between plants extract and glucantime p0. Ace inhibitors like perindopril and captopril and angiotensin ii receptor blockers like losartan had neuroprotective effect in the striatum and the sn in rats receiving 6hydroxydopamine 6ohda 11. Green synthesis of zincoxide nanoparticles from peganum harmala. World journal of pharmacy and pharmaceutical sciences. Effects of a triplex mixture of peganum harmala, rhus. Alkaloids with allelopathic activity are not as wellknown as other allelochemicals.
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